Questions and Answers
Questions and Answers
At this point, I’m starting to get a better idea of my “research question” for the summer, or in other words, what I am trying to answer through my research and experiments here in the Blobe Lab. I think it could go something like:
“In endothelial cells, how do various stimuli, in the form of ligands, affect the nuclear translocation of “Protein X” in the presence and absence of the TGF-Beta pathway co-receptor, endoglin?”
If you don’t understand my question, check out some of my earlier posts. I think it’s still too early for me to have any definite hypotheses, partly because Nam and I are going into territory that really hasn’t been explored before. There are also a number of experimental parameters we will need to play around with before we get major results. I’ll have to spend a LOT more time running my translocation assays and looking under the fluorescent microscope before I start to see any major trends. But that’s science I guess. Patience is key.
Here's a picture of some endothelial cells from another nuclear translocation assay I ran the other day. Again, I'm staining for the translocation of "protein X". This time, I used a fluorescent green antibody tag:
“Patience is something you admire in the driver behind you, but not in one ahead.”
-Bill McGlashen
