Uh Oh...Back to the Lab Again
Good news! I’ve figured out what went wrong with my immunofluorescence last week. It turns out that the reagents I used, in particular the Triton-X detergent I used to open up the cell membranes (so that fluorescent antibodies can enter the cell) were a bit old. After making a new batch of…well…everything, I got my signal back! I’m back in business, and back on track. Now that I’ve got the kinks in the technique worked out, I can really start going full swing into taking more pictures of the endothelial cells and gathering more data. Specifically, I want to get a rough estimate of how many cells, in the presence of certain stimulants, undergo nuclear translocation for “protein X”. In other words, time to start counting cells.
Here’s another picture of some endothelial cells I cultured…I think I might have put too many cells on the cover slip…‘Til next time.
"I don't know the key to success but the key to failure is to try to please everyone."
Bill Cosby
