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DNA is Hot

Posted by Olutoyosi Oyelowo on 2009-07-05

Hello World,

So, every weekday for the past three weeks, I have been going to the Center for Human Genetics. I have walked unto the fourth floor, put on my cooking apron, and oven mits (gloves).

I prepare the special of the day: two plates of Sephadex with a side of Mastermix for my PCR. What kind of meal is this, you ask?

It's the meal of a lifetime. It's what makes my summer reseach project. I am helping my lab with a project they have been working on for the past two years. This project revolves around a family with an hereditary kidney disease--Proteinuria.  So, we have been sequencing much of the DNA from the affected individuals of this family. We are now trying to identify where the mutation is located. We analyze many various single nucleotide polymorphisms (SNPS) and splice each exon.

Though many people might not like running PCR's or using the Sequencher computational program, I love it. And can't get enough it. It's delicious which is why I continue to prepare such dishes everyday. Sometimes, my results are burnt and useless, but other times they are crisp and practically perfect. Talking about it makes me crave it. Ah, I cannot wait until Monday.

I also cannot wait until the Poster Session so that I can give the world samples of my Reseach Project Plate!

Until Later,

ooo11

2 comments so far

Posted by Hetali on 2009-07-05
haha. really yummy. are you looking at a particular portion of the project? what conclusions are you yourself going to draw from your PCRs?
Posted by Toyosi on 2009-07-10
Yes, thanks for pointing that out. I managed to get carried away. I was distracted by the ideas of cooking and food. ;D So, my portion of the lab centers around trying to find the cause of the familial hereditary disease. My lab did some genome-linkage analysis and refined the search to only one chromosome--chr2. There are specific genes that play a huge part in the physical integrity of kidney functions. One gene (my focus) is GPR-75, the G-protein coupled receptor. Hence, I sequence the gene and PCR the DNA of the affected individuals. From this work, I hope to analyze the sequences and discover possible mutations. Or, at least, gain a better understanding of Genetics.