Mission Complete?
So, believe it or not, my own "project"/experiment is actually done. Well, not in the sense that there's nothing left to do or improve, but all the data that I wanted and could get by the end of Howard Hughes is already out. From here on out, it's either improving my results--mostly tinkering with antibodies and procedures--or starting on something new. I'm still on the edge of excitement right now though: my last stain (the only one I actually did well) turned out beautifully. I've made so many mistakes already, you guys would be appalled if you knew. Good thing I corrected everything, because these were the last cells of this cell line, so I was under lots of pressure to get it right. I think I will be getting another batch of cells from a different cell line (one that we can actually produce, whew) to culture and stain.
The results everyone will see are about 1/4 of the results I could have obtained, had I made less mistakes. But I'm glad I learned so much from them. =)