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Mis expectaciones

Posted by Allyson Morton on 2009-06-18 - one comment

So, I'm on Week 2 (Day 9) of the Howard Hughes program, and already I've learned so so so much. On day 1, I didn't learn much of anything - I just feverishly copied down everything Cathy (post-doc mentor) said (this still happens on occasion, but not too often). Now, I'm starting to get the hang of things, and I'm also doing more things on my own.

This summer, I want to grow more confident in my knowledge of molecular biology and cancer. I hope to be able to come into the lab in the morning and know exactly how I'm going to spend my time that day. I want to lose the feeling of constantly being scared that I'm about to mess up something.

Though I've been reading a lot of papers, it's been very hard for the information within to really "sink in"; as a result, it's hard for me to keep the big picture in mind. Whether this requires more studying on my part or just continued immersion in the lab atmosphere, I don't know - but by the end of the summer, I want to be able to talk about my research and the surrounding scientific principles (the TGF-beta signalling pathway and how it relates to colon cancer, the various parts of the TGF-beta family, etc) with confidence and ease, as well as be able to explain WHY I'm doing all the things I'm doing, instead of just doing them because I know they have to be done.

I hope I come out of this program with a plan. Throughout freshman year, I kind of went through the motions without having a goal or a plan - I just knew I wasn't pre-med (this undoubtedly helped my stress level). However, it isn't good not to have a goal, because without a goal, there is little motivation to do anything! So, whether I plan on working with TGF-beta in the future or if the work I'm doing now leads me somewhere else, either way I want to know something by the end of these eight weeks.

Me & My Lab

Posted by Allyson Morton on 2009-06-12 - no comments

Welcome to the Blobe Lab! This is in the LSRC on the 3rd level. It's kind of randomly situated with some IGSP labs and pediatric cancer labs. We have a fridge and a microwave for food!!!

 

 

 

 

 

 

 

 

This is a picture of me working at one of the microscopes. We made a scratch wound (basically taking a very thin pipette tip and scraping the bottom of a small well - the cells are stuck to the bottom) and are looking at the progress the cells make in "closing" the "wound." This is analagous to studying cell migration which is analagous to studying the ability of cells to metastasize in cancer.

 

 

 

 

 

 

 

 

This is me doing stuff in the lab at our bench. I can't remember exactly what I was doing..

 

 

 

 

 

 

This is our bench, where Cathy (post-doc I'm working under) and I do a lot of our stuff! A lot of the equipment we use most often is there too, such as the stopwatch, the pipetmen, the electrophoresis tray, and the PBS wash.

 

 

 

 

 

 

 

This is my very modest work space. Since it's so freezing right there, and since one of the lab technicians was gone this week, I've been using her desk... but on Monday I will have to go back here!

Tagged: Blobe-Lab, pictures

Hi guys! (v. 3)

Posted by Allyson Morton on 2009-06-11 - no comments

So, the first time I did this it got deleted thanks to me trying to edit a hyperlink..)

Hello everyone, my name is Allyson Morton and I'm another addition to the Howard Hughes Research Fellowship. I hail from the sunny state of North Carolina, where humidity is almost always 100% (I have a lot of opinions about the weather around here but I will try to keep them out of this blog). I've been a Duke fan for all my life - where I'm from, you either love Duke or you hate it, it seems - and I'll be a sophomore here this fall.

For this program, I'm working in the Blobe Lab along with another research fellow and friend, Arun Sharma (we both lived in the same dorm in freshman year - go Alspaugh!). More about the Blobe Lab can be found here: myprofile.cos.com/gblobe. I'm working with Cathy Gatza, a post-doc, and I'm having a lot of fun already, though working all day is definitely an adjustment. I've also interviewed both Cathy and Dr. Blobe and I'll be posting the interviews a bit later, as well as some pictures of my lab!

In the Blobe Lab, I'll be working with colon cancer and the role of TBRIII (one of the 3 receptors to TGF-B) in regulating and mediating cell migration (which is what metastasis is - cancerous cells deciding they've gotten bored of your lung or breast or colon and deciding they'd like to try out your brain or bones instead. Warning: This is bad.). The cool thing about colon cancer and RIII is that RIII shows opposite effects in colon cancer as compared to most other cancers. In breast cancer, for example, restoring RIII expression (as it is usually lost in breast cancer cells) inhibits tumor invasion, angiogenesis, and metastasis. The opposite is true for colon cancer - it promotes cancerous activity.

Anyway, that's all for now. Look for my other blog posts very soon!
 

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