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Refinements for the Interview with a Principal Investigator

Posted by Claire Deahl on 2009-06-29 - one comment

First off as Kriti very nicely pointed out P.I. does not stand for Personal Investigator, but Principal Investigator. In general it is important and polite to get title's right. I hope that Dr. Allison is not upset by this mistake. A Principal Investigator is a lead scientist for a specific research project. A Personal Investigator is something I believe I made up, (I think that it is a reformation of Private Investigator, but who knows?)

An introduction of my mentor:  Her name is Dr. Allison Ashley-Koch, she works in the Center for Human Genetics at Duke University. She is also an Associate Professor in the Section of Medical Genetics, Department of Medicine at Duke. She is a P.I. for several different projects in the lab, including: Neurol Tube Defects, Healthy Pregnancy Study, ADHD, Sickle Cell Disease, Multiple Sclorosis. Her intrests also include psychiatric disorders inlcuding Autism, Bi-Polar Disorder and Uni-Polar Disorder.

Marcy Speer was the previous director of the Center of Human Genetics and chief of the Division of Medical Genetics, and before that one of Dr. Allison's mentors in highschool, and part of college.

If there is anything else that doesn't make sense, or questions you may have, please ask!  Thank you Kriti!!!

Interview with a Principal Investigator (not a vampire)

Posted by Claire Deahl on 2009-06-26 - 2 comments

The following is a parphrasing of the interview:

How did you start? (which turned into a condensed story of how Allison got into and followed genetics into a career)

It began with an early intrest in genetics, beginning in a ninth grade biology class. This intrest then influenced her to apply to North Carolina School of Science and Math. At the time they offered a genetics course (I don't know if they still do), which she took her junior year. Also at Science and Math they had a special projects or focus week, a classmate's dad was a geneticist. For a week Allsion got to job shadow different people in the genetics department at Duke, including a molecular biologist and a genetic councilor. Her senior year she took a mentorship course at NCSSM, where she, using the contacts from the previous year, recieved course credit for working in the genetics field at Duke.

Then began college at UNC Chapel Hill, and she still kept in touch with the Human Genetics at Duke. At Chapel Hill she worked on a project with flies. She had many issues keeping the flies alive, separating the males and females, and often would come home covered in flies. From this Allison decided for sure that this was something she did not want to do, which is really just as important as deciding what it is one wants to do. She moved on to get a PHD at Emory in Atlanta. Here she did both lab or molecular work and statistical analysis. She liked the statistical analysis work the most. Her dissertation was on the Fragile X Syndrome, which is a gene that causes mental retardation.

From here she moved on to working as a post-doc at the Center for Disease Control and Prevention (CDC). She stayed here for a little over a year, and learned a lot about the public health side of genetics, including the ethical, legal and social aspects. But after a year she left to continue the rest of her fellowship at Duke in the Center for Human Genetics (it all just came back full circle). She actually ended up working for the same women who were her mentors during the NCSSM mentor program. During the two and a half years she spent at Duke, she worked on a number of projects including sickle cell disease, autism and neuro muscular disease. Very sadly Marcy passed away in 2007 and Allison inherited her projects, then from here the Healthy Pregnancy study began.

 

What is your favorite study/what do you like about any of your studies?

It was hard for her to choose which one was the best, much easier it was to choose her least favorite project- the flies mentioned afore. Healthy Preganancy is a very interdisciplinary study. It arose from Allison's intrests in neuro development and the health disparities involved. This disparities also arise from social and enviromental factors, which makes it include a lot more than genetics.

 

What is the hardest part?

Budgeting. This is because they never trained her for budgeting in grad school, and it's really complicated budgeting. It includes thinking of the projects all together, in short term and long term ways. Lots to think about.

Also she found mentoring a difficult thing, once again because it is not taught. It is really important to teach others and to do it well, communication can be difficult.

 

If you were not here in science, what would you be?

I would be in the medical field as a pediatrician. If not in the medical field, then teaching. A science teacher for elementary or middle schoolers.

 

What do you do in your freetime?

Take care of the family mostly. She has two boys Nathan, age 7, and Justin, age 4. Other than that she likes live music and going to the movies. Music includes mostly rock, including Lenny Kravitz, No Doubt, Gwen Stefani, Coldplay, Def Leppord and Journey.

 

Ever made a mistake?

Allison has an ethanol fire from college and a radioactive spill from grad school under her belt of mishaps. But other than that just little things like mixing up the gels so that the DNA runs off the wrong way.

 

Is it ever boring?

She doesn't find it boring but she says she'll get stuck a times.

 

Advice to Highschools/Howard Hughes students/anybody who doesn't know what to do next:

She says that it is most important to try a bunch of different things. Get Exposure! Finding out what it is that you don't want to do is just as important as knowing what you do want to do.

 

Extra information:

Current funded projects that Allison heads: Neurol Tube Defects, Healthy Pregnancy Study, ADHD, Sickle Cell Disease, Multiple Sclorosis

Unfunded projects: Muscular Dystrophy

Side Physcological projects (which I don't recall if they are funded or not...): Autism, Bio-Polar Disease, Uni-Polar Disease and Schizophrenia

 

 

 

P.S. I realize now that I switched from second and third and first person in my writing of the interview, just kind of ignore that, I think that the idea is clear enough....
 

Tests and Taqman Plates

Posted by Claire Deahl on 2009-06-21 - one comment

Okay, so for those who don't know part of the Howard Hughes program is that I get to work in a lab on part of a research project. My lab is in the Center for Human Genetics (CHG), which is located a ways away on the medical side of Duke's campus, but I don't mind because I get an ID badge. My mentor is Dr. Allison Ashley-Koch, who runs several different studies in the CHG. The study that I am a part of is called Healthy Pregnancy, Healthy Baby.

Monday and Tuesday (and part of Wednesday) were spent setting up my computer, Duke ID and a taking a bunch of protocal test. For my lab, Helen (an awsome Howard Hughes student who's in the same lab as I am) and I had to take 17 IRB/protocal/safety/silly tests before we could touch anything in the lab. YUCK! We even had to take one on radioactive material safety and disposal, but there are no such radioactive materials in my lab or building....

After this, Mike (a lab technician who's showing me around) gave me an introduction to a couple of machines in the lab, including the Biomek FX (which sounds a lot more intimidating than it actually is). The Biomek takes DNA from a master plate and then puts tiny amounts of it into Taqman plates. Taqman plates are rectangular pieces of plastic with lots and lots of tiny, little wells, the plates are then used for running reactions. After the Taqman plates are filled with DNA, the DNA is left to dry (after being put through a centerfuge).

The next step is to fill the Taqman plates with a solution using pipettes so that a reaction can be run and recorded. Part of my job is to make this solution using water, assay mix and a master mix. After I make the solution I get to use a multi-channel pipetter to fill in the wells. The multi-channel pipetter has eight tips to pipet the solution into at a time. I practiced this on Friday, and it is actually harder than it looks to line up all the tips into the wells at the same time, but I've kinda gotten the hang of it. Luckily, when I miss a well I can fill it up with a single channel pipet, so no worries there.

After the Taqman plates have been filled they go into another machine that runs the PCR reaction and then another which records the data from the reaction. Later I will learn how to interpret this data using statistics and a computer.

Til then I'll be running Taqman plates and PCR reactions.

Tagged: Taqman-plates
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