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Why I am at Duke, and not at the beach.

Posted by Lisa Grossman on 2009-06-18 - no comments

So everyone has reasons for participating in summer research, or else we would have decided to spend our summers at the beach instead of applying for Howard Hughes. Contrary to popular belief, there are many reasons why one might choose a summer research job over a summer at the beach. Maybe you want to decide whether you like research or not. Maybe you enjoy the intellectual stimulation of doing research. Or maybe you simply think that summer research will look good on your resume.Here are some of my reasons for participating in summer research, and what I hope to get out of my summer experience. A lot of them are pretty standard, but if I tried to make up something cute, original, and funny, it probably wouldn't be as true. These are without a doubt the most important goals I have for the summer.

My expectations about this summer are that I will...

1. Learn more about virology and the scientific problems the lab studies. If I fully understand what I’m studying, in depth, I will make helpful insights and good decisions in lab. Furthermore, solid background knowledge in virology and immunology will help my lab career later on.
2. Gain procedural knowledge. How do you pipette? How do you use a FACS machine? How do you grow a cell culture? These skills are best learned through experiential education, so I’d better take advantage of my Howard Hughes internship by learning as many lab techniques as possible!
3. See how I like working full-time in a laboratory. Is lab science something I want to do for a job later? Though I will not decide for or against becoming a scientist during just this one internship, Howard Hughes can definitely give me a good idea of what a full-time lab job is like.
4. Get busy, work hard, and come up with some good data. A lab internship is no fun at all if you are bored and don’t have enough to do! I’d much prefer to spend what little time I have working hard on my experiment, studies, and lab technique.
5. Keep a thorough, up-to-date lab notebook. I’ve had problems with this in the past, as I tend to not write down my exact experimental procedures in full. An incomplete notebook is poor science, and I must learn to keep a better record.
6. Get to know my lab mates.
Everybody in my lab is incredibly interesting and intriguing, and each has their own fascinating story. Furthermore, every one of them knows so much about science! The more I get to know my lab mates, the more I learn from them and enjoy their company. I'd also love to get to know my fellow Howard Hughes researchers, who are just as funny and interesting.

Tagged: Luftig, virology

MONO VIRUS! Ok, now that I have your attention...

Posted by Lisa Grossman on 2009-06-13 - 2 comments

Hello! I’m Lisa Grossman, from a small town in Colorado. There isn’t much research going on where I’m from (although there is a lot of mountain climbing!) so I’ve come to North Carolina to participate in Howard Hughes this summer.

I’m working in the Luftig lab, from the Molecular Genetics and Microbiology Department, which studies Epstein-Barr Virus (EBV). Several of you probably know this virus up close and personal, as it causes infectious mononucleosis. However, EBV is also a co-factor for several cancers. EBV induced cancers include Burkitt’s Lymphoma, the most common childhood malignancy in sub-Saharan Africa, and nasopharyngeal carcinoma, one of the most common cancers in eastern Asia. I work with the cancerous side of EBV, studying how the virus induces B cell proliferation in vitro.

I absolutely love my lab and my lab mates, including my amazing mentors Pavel Nikitin and Micah Luftig! To those of you interested in virology, you can check out my lab at http://mgm.duke.edu/faculty/luftig/ and Duke virology at http://mgm.duke.edu/virology/.

This week I’ve been ordering materials for my experiments, setting up other experiments, running preliminary experiments for my project, and culturing EBV, among many other tasks. I’ve learned to be very careful and clean so I don’t accidentally infect my cells/myself with any dangerous particles!

Here are some pictures...

A picture of research drive taken while walking to lab. The CARL (Clinical And Research Laboratories) building, which houses the lab, is up about three blocks.

Eleonora putting cells in the hood! The incubator is directly behind her.

The PI, Dr. Micah Luftig.

Jing standing next to the cryogenic container with liquid nitrogen, where we flash freeze cells.

Chris, the awesome lab technician, cleans the inside of the hood where he's working.

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